Hi,
First of all , no matter how did you assign the permission to users , please confirm the 'apply to 'option was set to :this folder ,subfolders and files as following under advanced security tab :
If there are any possible that the permission was changed unexpected ,i would suggest you enable the audit policy for the files. For more details you can refer to the following link:
https://techexpert.tips/windows/audit-deleted-files-on-windows/
(In this link, the audit policy is for delete operation, you can change that to change permissions)
After the audit policy was enabled , you can try to clear the permission and assign the read permission again , and audit who add the create permission
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