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Hi @SAT,
it has been resolved now. issue was related to system mailbox which were missing.
Great to know that it works now and thanks for the share! Since the Microsoft Q&A community has a policy that "The question author cannot accept their own answer. They can only accept answers by others", I'll repost your solution in case you'd like to "Accept" the answer.
[Admin accounts cannot access ECP, Exchange 2019 CU12]*
Issue Symptom:
"We migrated from Exchange 2010 to Exchange 2019 CU12. But none of original Org Management access accounts get access the ECP. We all get an http 500 error. I created a brand-new account and that works fine."
Resolution:
"it has been resolved now. issue was related to system mailbox which were missing. I used the following command which is mentioned the article https://social.technet.microsoft.com/Forums/en-US/b9a99e81-2e0d-4d26-b408-1f78c66946e2/administrators-cannot-login-to-ecp and it works for me."
get-aduser -filter "Name -like 'SystemMailbox*'" -Server xxxxxxx -Property Mail | ? {$.mail -eq $null} | Foreach {Enable-Mailbox $.DistinguishedName -Database xx-DAGN1-xxx001}