Hi @Bibin GV ,
Welcome to Q&A forum!
You mean, SharePoint 2016 cannot be opened in Chrome, but it can be opened in IE, right?
Or is there a problem in both browsers, but the situation is different between the two browsers? Have you tried other browsers?
For example, Fire Fox. Are the results different?
According to the picture you provided, Access Denied is displayed, which may be an issue with account permissions. Please confirm the permissions of the account, or try changing the account with permissions.
If the answer is helpful, please click "Accept Answer" and kindly upvote it. If you have extra questions about this answer, please click "Comment".
Note: Please follow the steps in our documentation to enable e-mail notifications if you want to receive the related email notification for this thread.