Here are similar issues for your reference:
https://social.technet.microsoft.com/Forums/office/en-US/15dba2d5-152d-4e27-9a19-31df8ed3c93e/is-it-possible-to-lock-down-the-vtibin-and-layouts-pages-with-a-wildcard-option?forum=sharepointadminprevious
https://sharepoint.stackexchange.com/questions/167264/restricting-access-to-contents-in-vti-bin-for-both-authenticated-as-well-as-a
As trevor said, many pages may need to call these services. It is not recommended to restrict access. If this is necessary, you may only add configuration for each subsite.
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