Hello Laman,
Have you check if the path of audio is ok? you can test it with the full path or absolute path:
path = 'C:/path_to_project/Audio/......'
Maybe another issue is the file permission of the audio file, to make some check apply everyone...
Also you have to pip the correct libraries (I assume that you have already done that).
Last but not least close all the programs that use the file due to file lock issue.
If you need something further pm me.