https://support.microsoft.com/en-gb/topic/kb5014754-certificate-based-authentication-changes-on-windows-domain-controllers-ad2c23b0-15d8-4340-a468-4d4f3b188f16 is your reference.
If you have not applied the StrongCertificateBindingEnforcemen t registry key change to put into compatibility mode for unsecure certificates, no-one will be authenticated and no-one will be able to login.
https://www.reddit.com/r/sysadmin/comments/1im304c/strong_certificate_mapping_is_fully_enforced_from/ - reference.
Good luck!