Hello Jamie McFarling,
Thank you for posting in Microsoft Community forum.
Have you made any changes before the problem occurs?
You can try to restart the Domain Controllers during downtime and then check if it helps.
Here are two similar threads for your reference.
[8f4a-94ca-161-93c9] (microsoft.com)
[4ca6-9993-528-0d7e] (microsoft.com)
I hope the information above is helpful.
If you have any question or concern, please feel free to let us know.
Best Regards,
Daisy Zhou