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Dear Ryan,
Thanks for your updates and kindness.
From the latest updates, you encounter the same error message when you open it from the Excel application and Excel for the web.
If you can open other Excel files, I suggest you open them>Options>Save>Save files in this format and check whether .xlsx is selected. If no, select it and open the affected file again to see the result.
If the issue persists, the file may be corrupted. The next step may be to use some tools such as the Open XML SDK to further investigate the content of the file and see whether it can be repaired.
I suggest you post a new thread on the next support channel and send the file to the experts there to see if the file can be repaired.
Office Open Specifications: Microsoft Q&A
Here's a similar thread from there for your reference.
Invalid XLSX Diagnostics - Microsoft Q&A
Sorry for that our category may have limited resources on using the Open XML SDK to repair the file.
Thanks for your understanding and effort.
Sincerely
Cliff | Microsoft Community Moderator