Hi SZ,
Thank you for the update of the root cause. However, I want to provide a crucial piece of technical context for your future infrastructure planning. The limitation you encountered is specific to Server 2012 R2. Starting with Windows Server 2016, Microsoft re-architected ReFS to fully support Direct I/O on CSVs. In fact, on Server 2016, 2019, and 2022, ReFS is the recommended file system for Hyper-V workloads because it enables accelerated VHDX operations (such as instant fixed disk creation and block cloning for checkpoints) which NTFS cannot do.
Since you are running an environment where this limitation exists, sticking with NTFS is the correct stable choice to ensure you get the Direct I/O performance across your SAS fabric. Just be aware that when you eventually migrate this cluster to a newer OS version, you will not be bound by this restriction and should reconsider ReFS for its virtualization performance benefits.
I hope you've found something useful here. If it helps you get more insight into the issue, it's appreciated to accept the answer. Should you have more questions, feel free to leave a message. Have a nice day!
VP