Sorry no. This is not supported scenario.
We can encode the MKV file, but require that you only have a single audio track - unless you are trying to do some form of multi channel audio mapping, in which case look at this sample for that scenario.
https://github.com/Azure-Samples/media-services-v3-dotnet/tree/main/VideoEncoding/Encoding_MultiChannel_Audio
The service does not have support for the ASS file format.
If you need to add multiple subtitle tracks, you can upload .vtt or .imsc1 side car files, but you would need to reference those in your player framework as a side-car subtitle file. Meaning, you will need some javascript logic to load them. We are working on some future features to more easily update the manifest when uploading side-car vtt or imsc1 track files for VOD delivery. That work is ongoing this semester and should be ready in Q1 of CY22.