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Hi @Tara
Thank you for posting your question in the Microsoft Q&A forum.
You’re seeing the 550 5.7.708 Service unavailable. Access denied, traffic not accepted from this IP error because Microsoft’s outbound mail protection is blocking traffic from your tenant or the IP address used by the integration. If you’re on a trial subscription, this is expected behavior until licenses are assigned.
As the Q&A Assist already mentioned, to resolve the issue, you’ll need to open a support ticket in the Microsoft 365 Admin Center and request an IP address exception for your tenant.
To open a support ticket, log in https://admin.microsoft.com/ with your admin account. Then choose Help & Support, briefly describe your situation and provide details like error message.

I hope this information is helpful.
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