This is a bug which may be akin to the legacy Jason Tab. Here is a temporary link to a document demonstrating the problem: https://www.dropbox.com/scl/fi/hm2wq4b5zzuq207530z9p/deleteme-TOC-direct-formatting-problem.docx?rlkey=4wi2ykieysnl054zz6qrgb3dz&dl=0

But about 2 weeks ago, I started seeing duff styles appearing in the style pane.
First, you have set your Styles Pane to show [direct] formatting as a style. What is showing is some direct character formatting, using Calibri Theme font. This is a setting in the Style Options dialog.

Second, your "+ Body (Calibri)" is a Theme font indicating that you directly formatted something in your TOC using that font. See screenshot below.
Whenever you update a Table of Contents it will re-apply the built-in TOC # styles.
If you want your TOC Level 1 entries to be red, modify that Style to be red. Then when you update, that color will be applied automatically. It looks like this has been done.
Note that there may be two showings for Calibri. If it is a Theme font, the first will be the Theme font and will say (Body) on the right. This will change if the Theme changes. Further down will be simply Calibri. That one will appear regardless of Theme settings so long as you have Calibri installed.

Resources:
I reproduced your problem to some extent and believe it is a bug similar to the Jason Tab.
I selected a space in a TOC 1 entry and applied the Theme body font. That is a different font from the font set in the TOC 1 style. I did the same with a TOC 2 entry. I am using Microsoft 365 Family, release 2603 on a Windows 11 Professional machine.

After updating the TOC, the direct formatting entry appears in the Styles Pane.
I can right-click on it in the Styles Pane and Select all instances.

If I select All instances and apply TOC 1 to the selection, the direct formatting entry disappears from the Styles Pane and the black paragraph marks change back to matching the color of TOC 1. If I then update the TOC, the direct formatting entry reappears and the paragraph marks change back to black.
If I then select all instances, only the paragraph mark at the end of the TOC 1 entries is selected.
If I reapply TOC 1 to that the direct formatting disappears from the Styles Pane but it will come back with an update of the TOC.
Here is the TOC before reapplication of TOC 1 (and after an update of the TOC). Note the paragraph marks!

Here is the TOC after reapplication of TOC 1.

If the TOC 1 did not include color, I would not even see this. I also did something similar with a TOC 2 entry.
In my opinion, this is a bug. I do not know whether or not it is recent. I know of no way to remove this from your document or template. The bug is the formatting reappearing in the form of those paragraph marks.
I did not see any change to the tab before the page number.
See one of my comments to one of Stefan Blom's answers where I speculate about this being akin to the Jason Tab bug. I believe Stefan knows more about that bug than I do.
I have modified the heading on your post to include the term Jason Tab in the hope that others will look at it too.
I cannot tell you why this is happening, only that it will not interfere with the appearance of your document unless you are typing inside the TOC.