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That's a really well-built case, and it pretty much rules out your side. Same client and same IP, with a clean 0.1s answer on one call and complete silence on the next, after TCP and TLS both succeed. That pattern usually means one unhealthy backend instance behind the regional gateway's load balancer, and your calls only fail when they land on it. Nothing in your VMs or network can fix that, so it needs Microsoft to pull the bad node.
- There's no supported way to pin a protected item to a different gateway. As you found, new vaults and re-enabling replication stay on the same regional endpoint.
- The closest supported workaround is private endpoints for the vault. With them, the agent talks to the vault over private IPs instead of the public gateway address, which changes the ingress path. Private endpoints can only be added to a vault with no protected items, so it means a new vault plus private DNS zones. It isn't guaranteed to avoid the faulty node, but it's worth a test on one VM.
- Meanwhile, crash-consistent points should keep coming, so your RPO is still covered. If the alerts are noisy, you can lengthen the app-consistent snapshot frequency in the replication policy for now.
- Open a technical support case for Site Recovery and attach exactly what you posted here: the per-VM counts, the five timed calls to the same IP, the UTC timestamps, the vault name, and ideally a tcpdump from one failing call showing the request leaving with no response. Ask for it to go to the ASR service team as a suspected unhealthy regional gateway node in East US. Evidence this clean usually gets routed fast.
Moderators here can also escalate it and will ask for your vault details by private message.
If this helped, please click Accept Answer so others seeing the same timeouts can find it.
References: https://learn.microsoft.com/en-us/azure/site-recovery/azure-to-azure-how-to-enable-replication-private-endpoints https://learn.microsoft.com/en-us/azure/site-recovery/azure-to-azure-troubleshoot-network-connectivity