Hi Annie,
Are you using Edge or Chrome?
Also, Audio Output is for Output aka Speakers. Microphone is the Input option.
For Microsoft Edge, go to this page and ensure MIDI and Microphone is set to allowed.
edge://settings/privacy/sitePermissions/allSites/siteDetails?site=https%3A%2F%2Fassessment.assessfirst.com
I look forward to your response and am eager to collaborate until we find a solution.