Hi,
First, check your USB controllers’ power settings by opening Device Manager, then Universal Serial Bus controllers, right-click each USB Root Hub, select Properties, go to the Power Management tab, and uncheck "Allow the computer to turn off this device to save power," then restart. This ensures Windows isn’t disabling your audio devices to save energy. Next, try plugging your microphones or audio interface into different USB ports directly on the motherboard, avoiding hubs, since some ports or controllers can fail to initialize USB audio after updates. Then, verify microphone privacy settings under Settings > Privacy & Security > Microphone to make sure access is enabled for the device and apps, as updates can sometimes block microphones at the OS level. Finally, if the above steps don’t work, use a System Restore to a point before the KB5065426 update to undo any driver or registry changes that may be preventing Windows from recognizing your USB audio devices.
Kind regards,