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I understand how frustrating it can be when you turn off grid-alignment, but Windows still puts everything you personalized back after a reboot. The Q&A Assist has given the right path for this, so I will just add some other suggestions for you to consider, in case they might help!
1 - Refresh the icon layout after changing the setting
- Right‑click the desktop > go to View > ensure to uncheck:
- Align icons to grid
- Auto arrange icons
- After arranging your icons, right‑click desktop > Refresh
- Right-click the Taskbar > click Task Manager to open it
- Find and right-click Windows Explorer (
explorer.exe) > Restart
2 - Temporarily turn off cloud sync app
- These tools sometimes may restore icons automatically.
- If you use OneDrive, GG Drive, iCloud, etc. , try disabling them to check.
3 - Disable icons for theme
- Right-click your desktop > Personalize > Themes
- Under Related settings > click Desktop icon settings
- Uncheck Allow themes to change desktop icons > OK.
4 - Repair Windows with reinstallation
- If this is not a native Windows behavior, some components might have been corrupted.
- Open Settings > System > Recovery > Fix problems using Windows Update
- Click Reinstall now > follow the onscreen instructions to reinstall Windows.
Disclaimer: This reinstallation can fix update issues without removing your files or applications. However, we always recommend that if you have important data, you should back it up.
You're not alone facing the same situation. I hope the additional suggestions above help with your issue somewhat.
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