Hello @Mahesh Aralelemath ,
Thank you for posting here.
Is there a possibility of defining all Windows 2003/XP to get authenticated from Windows 2008R2 DC only?
A: Usually, if Windows 2003/XP machines are in the same sites as DCs with Windows 2008R2 DC, then Windows 2003/XP machines will find Windows 2008R2 DC to authenticate firstly.
If you have 2008 R2DCs and 2016 DCs in your domain now, during the downtime, you can try to shut down 2008 R2 DC and keep 2016 DCs running if possible, then check if Windows 2003/XP machines can be authenticated by 2016 DC without any failure.
Hope the information above is helpful.
Should you have any question or concern, please feel free to let us know.
Best Regards,
Daisy Zhou