Hey,
Yes, it is possible to enable Data Deduplication for the drive, which is used for File Server. The following guide covers the configuration: https://robertsmit.wordpress.com/2018/02/21/clustering-fileserver-data-deduplication-on-windows-2016-step-by-step-sofs-winserv-refs-windowsserver2016-dedupe/
As for disks, it depends on which disks do you want to use for file server. Might also help with cluster configuration: https://www.starwindsoftware.com/resource-library/starwind-virtual-san-for-hyper-v-2-node-hyperconverged-scenario-with-windows-server-2016/
Cheers,
Alex Bykovskyi
StarWind Software
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