In this case, if you have used a third-party tool to modify the installation media and bypass the hardware requirements, you may be unable to re-enable the secure boot. You will need to contact the Rufus team on GitHub and check with them if it is possible to undo the changes or perform a clean installation of Windows using the official media from the Microsoft website.
You can contact the Rufus team by clicking "New Issue" on this link.
https://github.com/pbatard/rufus/issues?q=is%3A...
Note: This is a non-Microsoft website. The page appears to be providing accurate, safe information. Watch out for ads on the site that may advertise products frequently classified as a PUP (Potentially Unwanted Products).