You can use a dynamic named range that automatically adjusts itself as you add or remove entries in A1:A100.
- On the Formulas tab of the ribbon, click Define Name.
- In the Name box, enter (for example) MyList
- In the Refers to box, enter the formula =OFFSET($A$1,0,0,COUNTA($A$1:$A$100),1)
- Click OK.
Next, we'll make the data validation rule use the named range.
- Select B1 (the cell with the dropdown).
- On the Data tab of the ribbon, click Data Validation.
- In the Source box, enter the formula =MyList using the name that you specified above.
- Click OK.