Hello,
Thank you for posting in Q&A forum.
If "Use simple certificate selection" option is checked, NPS server will select the certificate used for 802.1x authentication automatically. However, it may bring some issue if it selects a wrong Root CA.
To further check this issue, you need to:
1.Check the certificate configured on the Lan connection.
2.Check the Root CA used for wired authentication in NPS policy on the server.
3.Compare the two certificate matches or not and if it's trusted on NPS server.
Hope this answer can help you well.
Best regards,
Jill Zhou