Try something like this:
private void button_tienda1_Click(object sender, EventArgs e)
{
label_boton_pulsado.Text = "You choose A";
drawLine = true;
Invalidate( );
}
bool drawLine = false;
private void pictureBox1_Paint(object sender, PaintEventArgs e)
{
if( drawLine)
{
Graphics g = e.Graphics;
//Configuration of the pencil
Pen greenPen = new Pen(Color.FromArgb(255, 0, 255, 0), 5);
//Draw
g.DrawLine(greenPen, 220, 370, 220, 290);
}
}