Hi ,
As far as I know, you can.
Joining AD domain (when routing is ok) is DNS dependent. Once you have your network segments routing between them, joining the domain requires the proper clients DNS settings. All of the PCs that will need to join the domain will need to point to a DNS server that can resolve the necessary AD related records.
Don't forget to add new subnet to the Active directory Sites and services.
Best Regards,
Candy
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