Server Response: 550 5.1.1 Not our Customer
This error usually means that the email address you were sending to doesn't exist. Remove any unsent messages from the outbox (below the account folders in the folder pane), then compose a new test message with a unique subject and send it to yourself (i.e. from your Comcast account to the same account). Post any new error message in your reply.
If the test message arrives, check the address you were originally sending to very carefully, then try again.