Hi CTM99, I'm Graeme, an independent adviser here to help.
The autoplay functionality might not work depending on how the ISO file was configured at creation, but I'll give you two other ways to play the DVD.
Firstly, see if you can play the ISO DVD using Windows Media Player. Here's a tutorial on how to do this, which should work just the same with a virtual DVD:
https://support.microsoft.com/en-au/help/15067/...
Otherwise, try downloading a DVD player from the Microsoft Store to see if that works. Here's an article that shows you how to get a trial version of Windows DVD Player:
https://support.microsoft.com/en-au/help/402721...
Please let me know how you go! Thanks!