The easy way to do this with 100% certainty is to calculate a hash value for the original set of folders, and a second hash value for the copied folders. Then compare the two hash values. If as little as 1 byte of data in the copy is different, the hash values won't match.
I use the MD5 and SHA Checksum Utility app for this. The technical name for this type of software is a checksum utility.
Comparing the space occupied on the disk doesn't tell you whether the contents of that space is the same. And it's misleading because the same files could occupy different amounts of space on two different disks, depending on how the data is laid out.
Back in the day, comparing hashes is something you would do whenever you transferred a large amount of data from one volume to another volume. However, today disk I/O has become very reliable. Today, people compare hashes mostly when they're downloading large files from the internet, and the hash is used to ensure that the file that you wanted to download and the file that you actually downloaded are the same. Microsoft used to publish the hashes for Windows ISOs, but they no longer do.