C$, IPC$, and ADMIN$ Network Share Access (Windows Server 2008 R2 Standard)

I'm trying to impliment some registry changes to allow elevated access to the $C, IPC$, and ADMIN$ shares for Nessus scanning purposes. I've implimented the following changes:

-HKLM\software\Microsoft\Windows\CurrentVersion\Policies\System\

• EnableAccountTokenFilterPolicy 1
• LocalAccountTokenFilterPolicy 1

-Winreg permissions

• HKLM\SYSTEM\CurrentControlSet\Control\SecurePipeServers\winreg
• Create a allow read all permission set for the winreg folder

-HKLM\System\CurrentControlSet\Services\LanmanServer\Parameters

• AutoShareServer 1
• AutoShareWks 1

I tested the changes in a lab environement and was able to good credentialed scan with the desired administrative privilieges. However, I do have some concerns before making the changes in the live environment, as the lab environment does not have end users attached to test if the changes will affect their access/normal use.

1. Do I need to disconnect the old drives that are inaccessible to the admin credentials or should I run the NetShareDel funtion on those shares?
2. Will disconnecting/deleting those shares have an impact to authenticated users active on the net?
3. Do I have to log out/restart the domain controller for those changes to take effect, or is there any other (less disruptive) method to have those shares regenerated with the desired access?
4. When the shares are regenerated after deletion/disconnection, will they still contain the same contents of the drive previously, just with adjusted permissions?

Any information would be greatly appreciated!

-Jon