Hello there,
You can transfer the FSMO role during business hours there will be no issue assuming that the health of both DCs are good and there is no replication issue.
The FSMO transfer is much more immediate. Make sure that the DCs are healthy and can communicate with each other. Even though the transfer doesn't have any downtime, make sure that you have proper change control in place, and i would recommend it done by the close of business hours.
You can find some useful links from this article https://social.technet.microsoft.com/Forums/en-US/5a490fd5-36dc-45a8-851e-00a599ba01d1/can-we-transfer-the-fsmo-roles-during-the-production-hours?forum=winserverDS
How to Migrate Windows Server 2008 R2 FSMO roles to Windows Server 2019 https://techcommunity.microsoft.com/t5/itops-talk-blog/how-to-migrate-windows-server-2008-r2-fsmo-roles-to-windows/ba-p/538377
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