Hi @Caspar - ABO ,
Based on my experience, normally Outlook usage won’t be affected when you change the Virtual directory URLs. Just make sure the DNS records for “mail.company.com” are added properly in your scenario. If you still have concerns on this, you can make the change at non-working hours.
Here is the result of running Test E-mails AutoConfiguration after I experimentally modified the internal Autodiscover URL. The original url was using the FQDN of my Exchange server, I changed it to “mail.contoso.com” and added the corresponding DNS record. I kept Outlook running during the whole process and didn’t experience any issue. The output of Test E-mails AutoConfiguration indicates the new Autodiscover url is being used successfully:
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