Hello Loganathan R,
Thank you for posting in our Q&A forum.
Q: Does that make any impact to windows machines in the term of Authentication or Any other things.
A: Before disabling MD5 via GPO, you had better check whether you have any old application or programs or old Operating System to still use MD5, if so, then you cannot disable it.
Hope the information above is helpful.
If you have any question or concern, please feel free to let us know.
Best Regards,
Daisy Zhou
============================================
If the Answer is helpful, please click "Accept Answer" and upvote it.