when there is no built-in Internal Web Application that is used in my AD Domain, is this builtin group membership still required?
IIS may have been installed as a requirement of some other software. WSUS for example. Open up the IIS manager and look at the defined web sites. What sites are running?
Look at the application pools. Are any of the accounts listed under the Identity column?
so if I remove the user from IIS_IUSRS builtin group membership, what will be the consequence when accessing the internally built Web App?
You must understand that we don't anything about the accounts that are listed in your IIS_IUSRS group, or where on your file system someone used that group for some purpose other than to grant IIS worker process accounts access to read the web content.
If the person who set up your web site (server?) did not understand the function of the IIS_IUSRS group, we have no way to know where that group was applied to file system security permissions. Thus we would have no way to know the impact of any change to group membership.
Who configured the server? Ask them why those accounts are in that group.
As I noted in first reply, according to the Microsoft documentation, there is no need for you to add any account to that group. When the IIS worker process starts up, IIS will add the token for that group to the account that the worker process executes as.
You need tell us something about the accounts that are listed in the group. Are these end user accounts? Are they software related accounts?