Is that a 403.2 error? If you uploaded a new dll to the site, check the security permissions and verify that they match the permissions on other files.
Implement IIS's failed request tracing and capture the 403 failure.
https://www.bing.com/search?q=iis+failed+request+tracing
When you double click on the .xml file it will open in Edge. In the settings menu, select "Reload in Internet Explorer mode" to view the data.