You need more information to analyze your problem, what is your request url? check if it is correct, are you using http or https? usually, this is a 404 error, you can use failed request tracking to view detailed error information.
Besides, you can also check below settings:
- Please check whether the certificate bound to the website has a matching private key
- Grant enough permission to the Application Pool that the application was using
- Reset IIS in cmd using
iisreset
command - Change port number in project properties
If this doesn't work, please post your web.config file of iis.
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