Hi Daisy,
Please refer below details:
Thank you for posting in Microsoft Community forum.
1.Based on the description "We have an issue where one application using the LDAPS VIP is unable to authenticate to AD.", what error message did you see when the LDAPS VIP is unable to authenticate to AD? - <<username>>: Hard error on read, OS error = 104
2.What application did you use? Microsoft application or non-Microsoft application? - Non-Microsoft
3.Can this application be authenticated properly before? If so, did the issue come up suddenly? Or is it a newly deployed application with LDAPS VIP that cannot be authenticated? - Yes, it was authenticating properly earlier. We rebooted the DCs which restored authentication as part of troubleshooting.
4.Did you install external SSL certificate on all the Domain Controllers? - Only 4 Domains controllers are being used behind the VIP for LDAPS. Certificate is installed on all 4 DCs
5.Where did you install certificate on Domain Controllers? - Both locations
Certificates- Local Computer
Personal container
OR
Certificates - Service (Active Directory Domain Services) on Local Computer
NTDS\Personal container
Thanks!