If your application already communicates with Application Insights over TLS 1.2 or higher, no action is needed. Upgrading your application’s TLS version does not necessitate migrating from the Application Insights SDK to Azure Monitor OpenTelemetry. So No, it will not impact
See:
https://learn.microsoft.com/en-us/azure/azure-monitor/app/opentelemetry-enable?tabs=aspnetcore
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Regards,
Abiola