No, you do not need to run the Wizard again.
You can simply change the MX record
https://learn.microsoft.com/en-us/exchange/decommission-on-premises-exchange
Assuming that you have already moved all of the mailboxes to Exchange Online, you can point the MX and Autodiscover DNS records to Exchange Online, instead of to on-premises. For more information, see External Domain Name System records for Office 365.
Make sure to update both the internal and external DNS, or you may have inconsistent client connectivity behavior.