Hi @Yuki Sun-MSFT ,
thank you so much for your help. Yes, i have added our domain to Microsoft 365 and created the new user as an active user. On your hint "Migrating the postbox" I searched how to do so for IONOS and finally found out that i never correctly added the mail-dns-entry for that domain correctly. So now its working all fine.
The error 500 had never occured again. Maybe it was an licence issue after adding it to the new user.
So again - thanks a lot.
Lukas