Using Classic Outlook on Windows for personal email, calendar, and contact management
Hello Ana,
Thanks for your post in Microsoft community. According to your post, we understand that you have configure SMTP relay to send emails from your application/software and had set up with SMTP client submission. For SMTP client submission method, O365 user account is required, so we would like to suggest you to check if you can login with the same credentials of that account on another device or Outlook web app. Check port settings are set as 587 or 25 and make sure TLS is enable at your application/software end.
You may refer this below article:
- How to set up a multifunction device or application to send email using Office 365: https://support.office.com/en-us/article/How-to-set-up-a-multifunction-device-or-application-to-send-email-using-Office-365-69f58e99-c550-4274-ad18-c805d654b4c4?ui=en-US&rs=en-US&ad=US
If we misunderstand your concern , we request you to kindly provide brief information about your requirement, so we can check further.
Thanks and regards,
Ankita Vaidya