A family of Microsoft relational database management systems designed for ease of use.
Jeanette's and Ken's answers are correct if your database was properly normalized, but I'm afraid it isn't. In a properly normalized database each question would be a record in a table. I'm afraid, what you have is a bunch of Yes/No fields in a table, each field representing a question. This will make analyzing the results of the questionnaire more difficult in the future. Also makes maintenance of the questionnaire more difficult. So I would urge you to think about redesigning your database in a more normalized fashion.
You will find an example of a properly designed questionnaire database here:
But to answer your question, just add the field. In a query add a column:
TotalYes: ABS(field1+field2+field3...)
As noted a Yes is stored as a -1 so the ABS() function turned it in a unsigned number)