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In the cells in your screenshot, the time in column I is before the time in column F, so the difference is negative.
Excel cannot display negative time.
Of course, the reason for this is that F is before midnight and I is after midnight.
The simplest solution is to use the date+time columns:
=G134-C134
If you prefer to keep on using columns F and I:
=MOD(I134-F134, 1)
(If Excel displays the result as a number, simply format the cells with the column as time)