Hello JohnJY,
Microsoft does not recommend the use of any type of fault-tolerant adapter or "Teaming" for the heartbeat. If you require redundancy for your heartbeat connection, use multiple network adapters set to Internal Communication Only and define their network priority in the Cluster configuration. Issues seen with early multi-ported network adapters, verify that your firmware and driver are at the most current revision if you use this technology.
No protocols other than TCP/IP should be enabled on the heartbeat adapter. Verify that all others are disabled (including such items as Network Monitor).
Considering the twist thresholds, it isn't mandatory after the Cluster threshold is updated. but it is advisable as a precaution to go for twist thresholds.
Hope this answers all your queries, if not please do repost back.
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Regards,