To turn SMB signing back on, you just reverse the settings with these two PowerShell commands:
Set-SmbServerConfiguration -RequireSecuritySignature $true -Confirm:$false
Set-SmbClientConfiguration -RequireSecuritySignature $true -Confirm:$false
Run them as Administrator, then restart your machine. This makes signing mandatory again, which is the default for newer Windows builds. If you want to go even further and enable signing (not just require it), you can also run:
Set-SmbServerConfiguration -EnableSecuritySignature $true -Confirm:$false
Set-SmbClientConfiguration -EnableSecuritySignature $true -Confirm:$false
That ensures both the client and server actively sign SMB traffic.