Hello @Kim Strasser ,
You should keep that option enabled since your app will run in compatibility mode on Vision Pro. It won't be a native visionOS app with spatial features, but it'll work as a 2D app in a floating window.
Visual Studio doesn't currently support the visionOS simulator for .NET MAUI projects, but you can use the visionOS simulator in Xcode if you're on macOS.
While this is a non-Microsoft website, it is Apple official document.
Happy holidays.