Hi,
I am not sure what you mean exactly but with a single application Insights instance you can create maximum 100 availability tests. If you want to use Connection Monitor instead the limits are the following:
Maximum connection monitors per subscription per region: 100
Maximum test groups per connection monitor: 20
Maximum sources and destinations per connection monitor: 100
Maximum test configurations per connection monitor: 20
Please "Accept the answer" if the information helped you. This will help us and others in the community as well.