@Phoebe Lu (Shanghai Wicresoft Co,.Ltd.) Thanks for your response!
- All five RDS VMs are in the same collection and the same GPOs apply to them.
- I assume there will be no difference between gpresult for affected users and other users, seeing as all of them have the same GPOs applied, but we will check it.
- No temp profiles are generated that I know of, since the RDS server is able to mount a UPD for the user (not knowing that it's an "empty" UPD instead of the "real" one). I will double-check this.
- The user habits are very similar, they all use software from a small set of applications that we support on the RDS server.
We are not yet sure whether it's something more of a configuration problem (GPOs, ...) or infrastructure (connectivity to AD or file server, some process interfering with the stored UPDs, ...).
We'll check your suggestions and continue our search, maybe we can find some useful events around the time the issue happens. In the meantime of course we'd be happy with any further suggestions :)