This might be helpful LINK
Issuing CA fail to start
Hi Everyone,
Trying to migrate 2 tier offline CA from 2012R2 to 2020 and encountered issues.
The root CA is okay but the enterprise issuing CA is not. After importing the database, the issuing CA fail to start with the following error:
The system cannot find the file specified. 0x2 (WIN32: 2 ERROR_FILE_NOT_FOUND)
Can anyone help? Thanks.
KHH
2 additional answers
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Limitless Technology 39,791 Reputation points
2022-01-27T09:46:48.34+00:00 Hello KHH
I would invite you to check the next link to ensure that all the permissions are configured correctly:
Most likely the issue is that it doesn't find the file due to a lack of access permission.
Furthermore, for the same issue, there is a previous thread with different instructions for troubleshooting. Check it here: https://social.technet.microsoft.com/Forums/en-US/107d431f-3003-4ab2-94f7-bed355d7d43d/active-directory-certificate-services-wont-start-error-100?forum=winserversecurity
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KHH 26 Reputation points
2022-01-28T14:36:50.753+00:00 Thanks, everyone,
Issue resolved. It was a permission issue.