Hello @Vaughn Minter ,
Thank you for posting here.
Is your domain on-premise AD domain or Azure AD domain?
If it is a on-premise AD domain, we can not set up two-factor authentication (TFA) to authentication. But maybe we can set up two-factor authentication (TFA) with third-part tool or method, we can search on the Internet for two-factor authentication (TFA) to authentication if needed.
If it is a Azure AD domain, we can set up Multi-Factor Authentication.
For more information about Azure Multi-Factor Authentication, we can refer to the link below.
How it works: Azure Multi-Factor Authentication
https://learn.microsoft.com/en-us/azure/active-directory/authentication/concept-mfa-howitworks
Best Regards,
Daisy Zhou