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Hi,
Once you have used the product key, you cannot use it again as it converts into a digital license. I would like to get more details about your issue.
Did you make any hardware changes that resulted in deactivation?
If so, have you tried running the activation troubleshooter to reactivate Windows?
Using the Windows Activation troubleshooter
https://support.microsoft.com/windows/activation/using-the-windows-activation-troubleshooter
I look forward to hearing from you to get the results of the troubleshooting.