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We are having this error when run to Web API. We already upgraded .NET Framework to 4.8v on Windows Server 2012 R2. Check TLs 1.2 is enabled.
What is binding web api? You are setting public or selfsign ca? Have you setting require certificate? And what is authentication method for application pool?
If your web api connect to sql you must configure correct support to use tls 1.2 on sql.
There was no TLS setting on SQL Server. I added to Registry.
It's HTTPS API, set by self sign CA, it requires certificiate. Certificate authentication method TLS 1.2.
Application Pool Authentication :
If you use selfsign ca it is required that it must be registered into local client ca folder. Selfsign ca requires to be trusted server-client otherwise you should see an authentication error.